A rapid antibody assay system for screening hybridoma cultures

A procedure for the rapid qualitative assay of large numbers of antibody samples is described which has been extensively used for primary screening of mouse spleen cell-myeloma hybrids making monoclonal antibodies. It is a handling modification of standard indirect immunofluorescence of immunoperoxidase techniques which involves in situ localisation of antigens in a fixed and dried monolayer of cells on plastic culture dishes. The method is quick and economical and does not depend on hazardous reagents or specialised detection equipment.