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Chemotaxis under agarose: dependence upon polymorphonuclear leukocyte density

T M Schaack, R H Persellin

    Journal of Immunological Methods
    |January 1, 1981
    PubMed
    Summary

    Polymorphonuclear leukocyte (PMN) density significantly impacts chemotaxis assays. Higher PMN cell density positively correlates with a higher chemotactic index, indicating cellular cooperation in immune responses.

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    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Chemotaxis assays, such as the Boyden chamber method, are influenced by cell density.
    • Understanding variables affecting chemotaxis is crucial for accurate immune response assessment.

    Purpose of the Study:

    • To investigate the impact of polymorphonuclear leukocyte (PMN) cell density on chemotaxis measured by the under-agarose assay.
    • To determine the critical cell density required for detecting chemotaxis.

    Main Methods:

    • Chemotaxis was assessed using the under-agarose method.
    • The chemotactic index was calculated by measuring migrated cell distance and correcting for random migration.
    • Varying PMN densities (cells/mm2) were tested.

    Main Results:

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  • PMN cell density significantly influenced the number of cells responding to chemotactic stimuli.
  • Chemotaxis was only detected above a threshold density of 1.8 x 10^2 PMN/mm2.
  • A strong positive correlation was observed between PMN density and the chemotactic index (P < 0.001).

    • Conclusions:

    • PMN density is a critical factor for reliable chemotaxis measurements using the under-agarose assay.
    • The findings support the existence of cellular cooperation in the early stages of the chemotactic response.