Detection of Residual penicillin in milk by sensitive enzyme immunoassay

A convenient and precise method by an application of a highly sensitive enzyme immunoassay of penicillin to detect the veterinary penicillin residue in milk was studied. The procedures of the assay to detect more than 0.01 microgram/ml of ampicillin in milk were developed. Five commercial milk samples were tested by the assay procedure, and no veterinary ampicillin residue was detected in any of them.