Specificity of acid RNase from HeLa cell lysosomes

The specificity of the lysosomal acid RNase [Saha, B.K., Graham, M.Y. and Schlessinger, D. (1979). J. Biol. Chem. 5951-5957] has been determined by 5'-end labeling and sequencing gel electrophoresis of rRNA. The enzyme has a predilection for the dinucleotide sequence ...NU..., which is cleaved into ...Np and HOU.... Sequences AU and GU sequences is enhanced by the presence of adjacent purine residues. The specificity remains unaltered after partial denaturation of the RNA.