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HLA standardization and proficiency testing in the Southeastern organ Procurement Foundation
Transplantation
|March 1, 1982
Summary
Interlaboratory cell exchanges improved Human Leukocyte Antigen (HLA) typing proficiency across Southeastern Organ Procurement Foundation (SEOPF) laboratories. Proficiency testing demonstrated benefits for regional HLA typing accuracy and antigen identification.
Area of Science:
- Immunogenetics
- Histocompatibility Testing
- Organ Transplantation
Background:
- Human Leukocyte Antigen (HLA) typing is crucial for organ transplantation success.
- Consistent proficiency in HLA typing across laboratories is essential for accurate tissue matching.
Purpose of the Study:
- To evaluate the progress in HLA typing proficiency over five years.
- To assess the effectiveness of interlaboratory cell exchanges for improving HLA typing accuracy.
Main Methods:
- Analysis of five years of data from Southeastern Organ Procurement Foundation (SEOPF) cell exchanges.
- Evaluation of 15 cell exchanges involving 60 cells to determine HLA antigen detection rates.
Main Results:
- Average detection rates exceeded 95% for numerous HLA-A and HLA-B antigens.
- Higher detection rates were observed for HLA-A locus antigens (85.8%) compared to HLA-B locus antigens (80.9%).
- False negatives were more common than false positives, with nonrandom errors for specific cross-reacting groups.
Conclusions:
- Interlaboratory cell exchanges significantly improved HLA typing proficiency within SEOPF laboratories.
- Regional cell exchange programs are effective for enhancing HLA typing accuracy and identifying provisional specificities.