Short method of processing animal tissues at 40 degree C for electron microscopy

Authors described a short-term method of fixation, dehydration and infiltration of animal tissues at the temperature of 40 degree C. The kidneys and livers of laboratory mice were used for experiments. Duration of described method was about 6 hrs. The quality of dehydration and embedding was good and there were no sectioning problems. Authors found no differences in basic ultrastructural features of tissues processed at 40 degree C against those processed at 20 degree C or 4 degree C. A short fixation at 40 degree C is therefore recommended, particularly for tissues from renal biopsies.