Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Distribution and changes in urease (EC 3.5.1.5) activity in Rumen Simulation Technique (Rusitec)

J W Czerkawski, G Breckenridge

    The British Journal of Nutrition
    |March 1, 1982
    PubMed
    Summary

    Urease activity in the Rumen Simulation Technique (Rusitec) system decreases over time but can be restored by urea infusion. Feeding frequency and diet type influence urease activity, with higher concentrations found in solid-associated microbes.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Metabolism of protein supplements studied by the rumen simulation technique (Rusitec).

    Archiv fur Tierernahrung·1985
    Same author

    Microbial fermentation in the rumen.

    The Proceedings of the Nutrition Society·1984
    Same author

    Microbial metabolism of 1,2-propanediol studied by the Rumen Simulation Technique (Rusitec).

    The Journal of applied bacteriology·1984
    Same author

    Effect of monensin on the fermentation of basal rations in the Rumen Simulation Technique (Rusitec).

    The British journal of nutrition·1981
    Same author

    Effect of monensin on fermentation characteristics of the artificial rumen.

    Applied and environmental microbiology·1980
    Same author

    A novel estimate of the magnitude of heat produced in the rumen.

    The British journal of nutrition·1980

    Area of Science:

    • Ruminant nutrition and biochemistry
    • Microbial ecology
    • Enzyme kinetics

    Background:

    • Urease activity is crucial for urea metabolism in the rumen.
    • Understanding urease distribution is key to optimizing nitrogen utilization in ruminants.
    • The Rumen Simulation Technique (Rusitec) provides a model for studying rumen fermentation.

    Purpose of the Study:

    • To investigate the distribution and dynamics of urease activity within the Rusitec system.
    • To determine factors influencing urease activity, including urea infusion and feeding patterns.
    • To compare urease distribution with other enzymes and microbial components.

    Main Methods:

    • Long-term experiments using the Rumen Simulation Technique (Rusitec).
    • Monitoring urease activity, ammonia, volatile fatty acids, protein, and DNA.

    Related Experiment Videos

  • Controlled urea infusion and dietary variations.

    • Main Results:

    • Initial high urease activity decreased over time, consistent with microbial dilution.
    • Urease activity was restored by urea infusion, showing a direct relationship with infusion amount.
    • Ureolytic activity was higher in solid-associated microbial compartments (compartment 2) than in free-floating microbes (compartment 1).

    Conclusions:

    • Urease activity in Rusitec is sensitive to urea availability and microbial population dynamics.
    • Dietary roughage and feeding frequency impact urease activity.
    • Specific urease activity is higher in the microbial suspension (compartment 1) than in solid-associated microbes (compartment 2).