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Mutagenic activity of sterilization indicators

T B Atherholt, G J McGarrity, J Louis

    Environmental Mutagenesis
    |January 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

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    Mutagens were found in black threads from hospital sterilization indicators and commercial sources. These mutagenic substances in sterilization indicator threads may affect experimental results, including mutagenicity studies.

    Area of Science:

    • Environmental Health
    • Toxicology
    • Microbiology

    Background:

    • Sterilization indicators are crucial in healthcare and research settings to ensure proper sterilization.
    • Black threads are commonly used in glass tube-type sterilization indicators.
    • Potential chemical contaminants in medical devices require thorough investigation.

    Purpose of the Study:

    • To investigate the presence of mutagenic substances in black threads used in sterilization indicators.
    • To determine if commercially available black threads also contain mutagens.
    • To assess the potential impact of these threads on experimental outcomes.

    Main Methods:

    • Acetone-methylene chloride extraction of black threads from sterilization indicators and commercial samples.

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  • Utilized the Ames Salmonella/microsomal assay to detect mutagenicity in the extracts.

    • Main Results:

    • Mutagenic substances were detected in extracts of black threads from glass tube-type sterilization indicators.
    • Mutagenic compounds were also identified in extracts of commercially sourced black threads.
    • Autoclaving fluids with these indicators may interfere with mutagenicity and other study results.

    Conclusions:

    • Black threads used in sterilization indicators can be a source of mutagens.
    • The presence of mutagens necessitates caution when using these indicators in sensitive research.
    • Further research is needed to identify the specific mutagens and mitigate their effects.