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Increased indole detection for Pasteurella multocida

K V Clemons, J L Gadberry

    Journal of Clinical Microbiology
    |April 1, 1982
    PubMed
    Summary
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    A new indole test using supplemented peptone broth quickly detects indole production in Pasteurella multocida. This method accurately identifies this common bacterial species within 24 hours.

    Area of Science:

    • Microbiology
    • Bacteriology
    • Diagnostic Microbiology

    Background:

    • Pasteurella multocida is a significant bacterial pathogen affecting various animal species.
    • Accurate and rapid identification of P. multocida is crucial for timely diagnosis and treatment.
    • Indole production is a key biochemical characteristic used in bacterial identification.

    Purpose of the Study:

    • To describe a supplemented 2% peptone broth medium for the detection of indole production by Pasteurella multocida.
    • To evaluate the efficacy of this medium for rapid indole testing.

    Main Methods:

    • Preparation of a supplemented 2% peptone broth.
    • Inoculation of the broth with 96 confirmed isolates of Pasteurella multocida.
    • Incubation and observation for indole production within a 24-hour period.

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    Main Results:

    • All 96 isolates of Pasteurella multocida tested were indole positive.
    • Indole production was detected within 18 to 24 hours using the supplemented peptone broth.

    Conclusions:

    • The supplemented 2% peptone broth is an effective and rapid method for detecting indole production in Pasteurella multocida.
    • This method facilitates the quick identification of P. multocida isolates.