Nervous system-specific protein D2 associated with neurite outgrowth in nerve cell cultures

Dissociated cerebral cells from fetal rat brain were grown in culture for various periods. After 12 days in culture the nervous system-specific surface membrane protein D2 reached both maximal specific concentration and maximal amount. Moreover, most of this D2 protein was in the perinatal form with high electrophoretic mobility. The amount of perinatal D2 protein possibly followed the amount of neurites in this system. D2 protein was also found in 2 neuroblastoma C-1300 clones: Neuro 2a and NB 41A3. By addition of gangliosides, Neuro 2a cells could be induced to differentiate and form processes, and D2 protein was significantly increased. However, in both differentiated and undifferentiated neuroblastoma cells D2 protein was present in the adult form with slow electrophoretic mobility. NB 41A3 cells were unaffected by gangliosides and D2 protein was not changed. Thus ganglioside treatment of Neuro 2a tumor cells was followed by a cellular response only partly similar to developmental events concomitant to differentiation of primary cells.