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Updated: Aug 11, 2026

16:43
Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells
Published on: February 18, 2014
Lysis of cell nuclei on perforated electron microscopic supports
Abstract:
The technique of gentle nuclear lysis for electron microscopic observation is suggested, which comprises adsorption of isolated nuclei on a positively charged pricked supporting film and sequential short-timed installation of the support with its back onto the filter paper moistened by lysing solution. The advantage of the method is slow, gradient-like, and to some extent regulated treatment allowing to visualize nuclear structures in their integrity.

