Hybridization between Brugia patei, B. pahangi and sub-periodic B. malayi

Virgin females of Brugia malayi, B. pahangi and B. patei were mated with males of species other than their own to determine whether they would hybridize. Microfilariae were produced in all but one cross (that between B. pahangi males and B. malayi females). Hybrid infective larvae, produced in mosquitoes and inoculated intraperitoneally into jirds, grew to adults but these were unable to produce microfilariae because hybrid males did not produce spermatozoa. Hybrid females were fertile and produced microfilariae when crossed with males of their parental species.