[Kinetics of urokinase hydrolysis of low molecular weight substrates]

The kinetics of urokinase hydrolysis of the low molecular weight substrates--S-2444 (Glu-Gly-Arg-pNA.HCl), N-benzoyl-L-arginine ethyl ester and acetylglycyllysine methyl ester have been studied. The kinetic constants for the hydrolysis reactions have been determined and the appropriate schemes have been proposed. The native enzyme is shown to be highly thermostable.