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Flow Cytometry-based Purification of S. cerevisiae Zygotes
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Directionality of yeast mating-type interconversion

A J Klar, J B Hicks, J N Strathern

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    This summary is machine-generated.

    Yeast mating-type switching is directed by a preference for specific silent loci (HML or HMR) as donors. This preference explains why some yeast strains switch mating types efficiently while others do not.

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    Area of Science:

    • Molecular Biology
    • Genetics
    • Yeast Biology

    Background:

    • The yeast Saccharomyces cerevisiae switches mating types via transposition of silent mating-type loci (HML and HMR) to the expressed mating-type locus (MAT).
    • Efficient switching is observed in strains with HML alpha HMRa genotype, suggesting directed, non-random switching to the opposite mating type.

    Purpose of the Study:

    • To investigate the mechanism underlying inefficient mating-type switching in yeast strains with the HMLa HMR alpha arrangement.
    • To determine the basis for preferential switching to the opposite mating type in some yeast strains and inefficient switching in others.

    Main Methods:

    • Utilized genetically marked HM loci to track donor locus preference during mating-type switching.
    • Compared switching frequencies and outcomes in yeast strains with different HML/HMR allele arrangements (HML alpha HMRa vs. HMLa HMR alpha).

    Main Results:

    • Strains with the HMLa HMR alpha arrangement exhibit inefficient switching (max 6%) due to preferential homologous MAT locus switches (MATa to MATa, MAT alpha to MAT alpha).
    • Alpha mating-type cells preferentially use HMR as the donor, while 'a' mating-type cells prefer HML, regardless of the silent loci's genetic content.
    • This donor preference leads to heterologous switches in HML alpha HMRa strains and homologous switches in HMLa HMR alpha strains.

    Conclusions:

    • The directionality of yeast mating-type switching is controlled by a preference for HML or HMR as the donor locus, not solely by transposing heterologous information.
    • Competition between HML and HMR loci for donating information to MAT mediates this switching preference.
    • Deletion of the preferred donor locus enables the inefficient donor to switch efficiently, supporting the competition model.