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Updated: Jul 29, 2026

Anti-Nuclear Antibody Screening Using HEp-2 Cells
Published on: June 23, 2014
Detection of antibody to carcinoembryonic antigen by the hemolytic plaque assay
Abstract:
A modification of the standard hemolytic plaque-forming cell assay is described. The conjugation of a soluble antigen, carcinoembryonic antigen, to the membrane of the sheep red blood cell was accomplished and used as the indicator system to detect antibody-forming cells. The specificity of the assay was determined by cross-reacting studies and competitive inhibition by antigen blocking.
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