[Assay of factors VIII and IX using an automatic system. Comparison of one- and two-stage techniques (author's...

The assay of antihemophilic factors is the best test for the diagnosis of hemophilia. It is also necessary to control the products for the substitutive therapy and for monitoring the treatment. The assays may be carried out by a semi-automatic technique. The comparison between this technique and the manual techique in two stages shows that the values obtained in one stage are higher than those obtained in two stages. The automatic method in one stage is sufficiently reliable for estimating the hemophilic factors.