Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

lac repressor-lac operator interaction: NMR observations

H Nick, K Arndt, F Boschelli

Proceedings of the National Academy of Sciences of the United States of America

|January 1, 1982

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Assessment of the impact of FcγRIIIA single-nucleotide polymorphisms on the efficacy of IgG1 monoclonal antibodies in patients with advanced gastroesophageal adenocarcinoma.

ESMO gastrointestinal oncology·2026

Same author

Correlation between angiotensin and acute kidney injury in patients with sepsis.

European review for medical and pharmacological sciences·2023

Same author

Measurements of Groomed-Jet Substructure of Charm Jets Tagged by D^{0} Mesons in Proton-Proton Collisions at sqrt[s]=13 TeV.

Physical review letters·2023

Same author

Predictive values of cervix length measurement based on transvaginal ultrasonography combined with pathological examination of placenta for premature delivery and correlation between premature delivery and infection.

European review for medical and pharmacological sciences·2023

Same author

Evaluation of Glazing and Polishing Systems for Novel Chairside CAD/CAM Lithium Disilicate and Virgilite Crowns.

Operative dentistry·2023

Same author

[Heterogeneity analysis of pancreatic cancer and identification of molecular subtypes of tumor cells based on CEACAM5, LGALS1 and CENPF gene expression].

Nan fang yi ke da xue xue bao = Journal of Southern Medical University·2023

The lac repressor

Area of Science:

Molecular Biology
Biophysics
Biochemistry

Background:

The lac repressor protein controls gene expression in Escherichia coli.
Understanding DNA-protein interactions is crucial for molecular biology.
The amino-terminal DNA binding domain of the lac repressor plays a key role in DNA recognition.

Purpose of the Study:

To investigate the structural changes in the lac repressor upon binding to lac operator DNA using NMR spectroscopy.
To identify the specific residues involved in DNA binding and discrimination.
To elucidate the mechanism by which the lac repressor distinguishes between specific and non-specific DNA sequences.

Main Methods:

Nuclear Magnetic Resonance (NMR) spectroscopy, specifically 19F NMR and 1H NMR.

Related Experiment Videos

Studying the aromatic residues (tyrosines and histidine) in the lac repressor's DNA binding domain.

Comparing NMR spectra of repressor-DNA complexes with varying DNA sequences.

Main Results:

NMR spectral changes indicate distinct interactions between the lac repressor and lac operator DNA.
Tyrosine residues in the repressor do not appear to intercalate into the DNA structure.
The amino-terminal domain demonstrates an independent ability to differentiate between specific operator DNA and non-specific DNA fragments.
Evidence suggests a 2:1 ratio of repressor amino-terminal fragments to operator in the specific complex.

Conclusions:

The amino-terminal domain of the lac repressor is sufficient for sequence-specific DNA binding.
The observed NMR spectral changes provide insights into the binding interface and mechanism of DNA recognition.
The lac repressor utilizes specific interactions beyond simple intercalation to achieve high-affinity binding to the lac operator.