Prostaglandins D2 and E2 are not regulators of amino acid release from rat cortical synaptosomes

Rat cerebral cortex synaptosomes synthesise prostaglandins, and analysis by gas chromatography-mass spectrometry revealed that of the prostaglandins quantified PGD2 (10.9 ng/mg protein) was produced in highest concentration. Treatment with high potassium or veratrine caused release of putative amino acid transmitters, but not of prostaglandins, and prostaglandins D2 and E2 were unable to stimulate release of amino acids. The release of putative amino acid transmitters, evoked by high potassium levels, was not inhibited by these prostaglandins. Prostacyclin receptors could not be identified on synaptosomes by radioligand binding techniques.