Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Factor XI antigen and activity in human platelets

G P Tuszynski, S J Bevacqua, A H Schmaier

    Blood
    |June 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Assessment of whole blood coagulation with a microfluidic dielectric sensor.

    Journal of thrombosis and haemostasis : JTH·2018
    Same author

    Plasma kallikrein activates the epithelial sodium channel in vitro but is not essential for volume retention in nephrotic mice.

    Acta physiologica (Oxford, England)·2018
    Same author

    Alzheimer disease is in part a thrombohemorrhagic disorder.

    Journal of thrombosis and haemostasis : JTH·2016
    Same author

    The contact activation and kallikrein/kinin systems: pathophysiologic and physiologic activities.

    Journal of thrombosis and haemostasis : JTH·2015
    Same author

    Prolylcarboxypeptidase independently activates plasma prekallikrein (fletcher factor).

    Current molecular medicine·2014
    Same author

    Recommendations for the Standardization of Light Transmission Aggregometry: A Consensus of the Working Party from the Platelet Physiology Subcommittee of SSC/ISTH.

    Journal of thrombosis and haemostasis : JTH·2013
    Same journal

    Tagraxofusp, Azacitidine, and Venetoclax in Blastic Plasmacytoid Dendritic Cell Neoplasm.

    Blood·2026
    Same journal

    Concurrent administration of BCMA and GPRC5D chimeric antigen receptor (CAR) T cells in advanced multiple myeloma.

    Blood·2026
    Same journal

    Single Cell Analysis of the Tumor Microenvironment Landscape Across the Disease Spectrum of Multiple Myeloma.

    Blood·2026
    Same journal

    Decentralized Clinical Trials in Hematology: the Promise and the Peril.

    Blood·2026
    Same journal

    How I Treat Chemotherapy-Induced Thrombocytopenia with Thrombopoietin Receptor Agonists.

    Blood·2026
    Same journal

    The Chaos of Choice in Large B-cell Lymphoma: A Call to Harmonize First-line Trial Design.

    Blood·2026
    See all related articles

    Platelets contain a unique form of factor XI (FXI) with coagulant activity and antigen. This platelet factor XI differs in molecular weight and structure from plasma factor XI, suggesting distinct functional roles.

    Area of Science:

    • Hematology
    • Biochemistry
    • Molecular Biology

    Background:

    • Platelets play a crucial role in hemostasis and thrombosis.
    • The presence and function of coagulation factors within platelets are areas of ongoing research.
    • Factor XI (FXI) is a key component of the intrinsic coagulation pathway, primarily found in plasma.

    Purpose of the Study:

    • To investigate the presence and characteristics of factor XI antigen and activity in washed human platelets.
    • To compare the molecular structure and antigenic properties of platelet-derived factor XI with plasma-derived factor XI.

    Main Methods:

    • Indirect immunofluorescence using a monospecific antibody to factor XI.
    • Staph A immunoprecipitation analysis followed by SDS-gel electrophoresis of radiolabeled platelet extracts and purified plasma factor XI.

    Related Experiment Videos

  • Immunoelectrophoresis of partially purified platelet factor XI.

    • Main Results:

    • Washed platelets exhibited significant factor XI-like coagulant activity and factor XI antigen.
    • Platelet factor XI demonstrated distinct molecular weights on SDS-PAGE compared to plasma factor XI (220 kDa vs. 160 kDa unreduced; 52 kDa vs. 80 kDa reduced).
    • Platelet factor XI showed complete antigenic identity but slightly different electrophoretic mobility compared to plasma factor XI.

    Conclusions:

    • Human platelets contain an intrinsic form of factor XI.
    • Platelet factor XI exists as a disulfide-linked tetramer of 52 kDa subunits, contrasting with the dimeric 80 kDa structure of plasma factor XI.
    • These findings suggest distinct structural and potentially functional properties of platelet-associated factor XI compared to its plasma counterpart.