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Computer-assisted monocyte esterase assay by flow-cytophotometry

L S Kaplow, E Lerner

    The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
    |July 1, 1977
    PubMed
    Summary
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    This study introduces a new computer-interfaced method for rapidly assessing intracellular monocyte esterase activity. The technique uses an azo-dye stain and light-loss signals to objectively quantify esterase levels in monocytes.

    Area of Science:

    • Immunology
    • Cell Biology
    • Biotechnology

    Background:

    • Monocytes play crucial roles in immune responses.
    • Assessing intracellular monocyte esterase activity is important for understanding monocyte function.
    • Existing methods for esterase activity assessment can be time-consuming and subjective.

    Purpose of the Study:

    • To develop and validate a rapid, objective method for quantifying intracellular monocyte esterase activity.
    • To utilize a computer-interfaced system for automated data acquisition and analysis.
    • To explore the patterns of monocyte esterase activity in mixed cell populations.

    Main Methods:

    • Interfacing a Wang model 2200 computer with Bio/Physics Systems Cytograf and Distribution Analyzer.
    • Employing an azo-dye technique to stain monocytes for nonspecific esterase activity.

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  • Measuring axial light-loss voltage signals from 1000 stained monocytes per sample.
  • Developing a custom software program to assign cells to activity groups and calculate an esterase score.

    • Main Results:

    • The developed method provides rapid and reliable data on relative intracellular monocyte esterase activity.
    • A scoring system was derived based on the distribution of monocytes into four activity groups.
    • Observed both Gaussian and bi-modal patterns of monocyte esterase activity, suggesting a potential dual monocyte population.

    Conclusions:

    • The described technic allows for rapid and objective assessment of intracellular nonspecific esterase activity in monocytes within mixed cell populations.
    • The observation of bi-modal patterns warrants further investigation into the existence of distinct monocyte subpopulations with differing esterase levels.