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Related Experiment Videos

Proline uptake in Candida albicans

N Dabrowa, D H Howard

    Journal of General Microbiology
    |December 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Candida albicans utilizes an active transport system for L-proline uptake at low concentrations. Mycelial cells exhibit higher L-proline transport rates than yeast cells, with diffusion also contributing at higher concentrations.

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    Area of Science:

    • Microbiology
    • Cell Biology
    • Biochemistry

    Background:

    • Candida albicans is a pathogenic fungus that can exist in both yeast and mycelial forms.
    • Understanding nutrient transport mechanisms is crucial for studying fungal growth and pathogenicity.

    Purpose of the Study:

    • To investigate the transport system for L-proline in Candida albicans.
    • To compare L-proline uptake in yeast and mycelial forms of C. albicans.

    Main Methods:

    • Assessing L-proline uptake at varying external concentrations.
    • Determining kinetic parameters (Km, Vmax) for L-proline transport.
    • Evaluating the effect of pH, temperature, and inhibitors on L-proline uptake.

    Main Results:

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  • L-proline enters C. albicans via a high-affinity, specific active transport system (Km = 0.1 mM).
  • Mycelial cells show a higher maximal transport rate (Vmax = 4 nmol/min/mg) than yeast cells (Vmax = 1.4 nmol/min/mg).
  • Diffusion contributes to uptake at higher L-proline concentrations (>0.1 mM).
  • Optimal transport occurs at pH 7.0 and 37°C.
  • Transport is inhibited by sodium azide and proline analogues (sarcosine, L-azetidine-2-carboxylic acid).

    • Conclusions:

    • C. albicans possesses a specific active transport system for L-proline, with distinct kinetic properties in its morphological forms.
    • The higher uptake in mycelial cells is not due to an inducible system.
    • These findings provide insights into nutrient acquisition strategies of C. albicans.