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Updated: Aug 13, 2026
09:27
Generation of Fluorescent Protein Fusions in Candida Species
Published on: March 4, 2017
Protoplast fusion hybrids of Candida albicans sterol mutants differing in nystatin resistance
Journal of General Microbiology
|January 1, 1982
Abstract:
Intraspecific protoplast fusion, induced by polyethylene glycol and Ca2+, was carried out in various pairings between auxotrophic nystatin-sensitive, ergosterol-producing strains of Candida albicans and their nystatin-resistant, ergosterol-less mutants of different origins. Nutritionally-complemented stable heterozygous diploid hybrids were obtained, which proved to be sensitive, semi-resistant or resistant to nystatin as a consequence of complementation or non-complementation for ergosterol biosynthesis. Dominant mutation control of the resistance was not found.
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