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Membrane properties and esterase activity of synovial lining cells: further evidence for a mononuclear phagocyte
Abstract:
Recent work in our laboratory with mouse radiation chimeras suggests that the macrophage type of synovial lining cell is derived from bone marrow. Further evidence for including this cell type in he mononuclear phagocyte system is now presented. Rosette formation with IgG coated sheep red cells, immunofluorescent staining for surface Ia antigens, and nonspecific esterase staining all show the presence in the synovial lining of a subpopulation of cells carrying these markers. The proportion of such cells in the lining is very variable, as is the proportion of 'type A' cells recognised with the electron microscope.
Insights
Synovial lining cells, specifically macrophages, originate from bone marrow and are part of the mononuclear phagocyte system. This study confirms their identity using specific cell markers.
Area of Science:
- Immunology
- Cell Biology
- Histology
Background:
- The origin and classification of synovial lining cells are not fully understood.
- Previous research suggested a bone marrow origin for macrophage-like synovial cells.
Purpose of the Study:
- To provide further evidence classifying synovial lining cells within the mononuclear phagocyte system.
- To identify specific markers for synovial lining cells of bone marrow origin.
Main Methods:
- Utilized mouse radiation chimeras to trace cell lineage.
- Employed rosette formation with IgG-coated sheep red blood cells.
- Performed immunofluorescent staining for surface Ia antigens.
- Conducted non-specific esterase staining.
Main Results:
- Demonstrated the presence of a subpopulation of synovial lining cells expressing markers consistent with the mononuclear phagocyte system.
- Observed variable proportions of these marker-positive cells and 'type A' cells (electron microscopy) in the synovial lining.
Conclusions:
- Synovial lining macrophages are derived from bone marrow.
- These cells belong to the mononuclear phagocyte system.
- The observed variability in cell markers and types suggests dynamic cellular processes within the synovial lining.