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[The antimycotica disc elution test: a new quantitative antimycotica test for yeasts (author's transl)]
Abstract:
The number of opportunistic yeast infections has increased during the last years. Therefore, testing of antimycotica has become important in terms of reliability and speed. Todays testing is essentially based on the disc diffusion susceptibility test and to a minor extent on the evaluation of the minimal inhibitory concentration. Two disc diffusion tests were compared. Both tests were shown to be practicable as a screening test. However, false positive results were obtained at a rather high percentage. Therefore, a new antimycotica test based on the disc elution test was developed and compared to a disc diffusion test. The resistance patterns for 5-fluorocytosine assayed by the disc elution test were in good agreement to the minimal inhibitory concentration. The test was shown to be adaptable for the imidazole-antimycoticas as well.
Insights
Opportunistic yeast infections are rising, necessitating rapid and reliable antifungal susceptibility testing. A new disc elution test shows promise, offering accurate results comparable to minimal inhibitory concentration for key antifungal drugs.
Area of Science:
- Medical Mycology
- Clinical Microbiology
- Antimicrobial Susceptibility Testing
Background:
- Increasing incidence of opportunistic yeast infections worldwide.
- Need for rapid, reliable, and accurate antifungal susceptibility testing methods.
- Current methods like disc diffusion tests have limitations, including high false positive rates.
Purpose of the Study:
- To compare the efficacy of two disc diffusion tests for antifungal susceptibility.
- To develop and evaluate a novel disc elution test for antimycotica.
- To assess the adaptability of the disc elution test for various antifungal agents.
Main Methods:
- Comparative analysis of two disc diffusion susceptibility tests.
- Development of a new antimycotica test based on the disc elution principle.
- Validation of the disc elution test against minimal inhibitory concentration (MIC) for 5-fluorocytosine.
- Evaluation of the disc elution test for imidazole-antimycotics.
Main Results:
- Both disc diffusion tests were found to be practical screening tools.
- A significant percentage of false positive results were observed with disc diffusion tests.
- The disc elution test demonstrated good agreement with minimal inhibitory concentration for 5-fluorocytosine resistance patterns.
- The disc elution test proved adaptable for imidazole-antimycotics.
Conclusions:
- Disc diffusion tests, while practical, yield a high rate of false positives.
- The novel disc elution test offers a reliable and accurate method for antifungal susceptibility testing.
- The disc elution test is a viable alternative for assessing resistance to 5-fluorocytosine and imidazole-antimycotics.