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[Morphological alterations in Escherichia coli and Serratia marcescens induced by cefotetan (author's transl)]
Abstract:
The effects of cefotetan (CTT, YM09330) and cefmetazole (CMZ) on the morphological changes of Escherichia coli NY-17 and Serratia marcescens IID 620 were examined with scanning electron microscope. 1) CTT and caused E. Coli NY-17 filamentous forms at 0.024 microgram/ml but CMZ showed same morphological changes at 0.2 microgram/ml which is about 10 fold higher concentration than that of CTT. Spheroplast like structures and cell lysis were observed at lower concentrations than those of CMZ. 2) In case of Serratia marcescens IID 620, filamentous forms, spheroplast like structures and cell lysis were also observed at lower concentrations than those of CMZ. The action of CTT against the both strains was bactericidal.
Insights
Cefotetan (CTT) demonstrated superior efficacy over cefmetazole (CMZ) in inducing morphological changes and cell death in Escherichia coli and Serratia marcescens. CTT proved bactericidal at lower concentrations, highlighting its potent antibacterial activity.
Area of Science:
- Microbiology
- Pharmacology
Background:
- Antibiotic resistance necessitates the development of effective antimicrobial agents.
- Cephalosporins are a class of beta-lactam antibiotics widely used to treat bacterial infections.
Purpose of the Study:
- To compare the morphological effects of cefotetan (CTT) and cefmetazole (CMZ) on Escherichia coli and Serratia marcescens.
- To evaluate the bactericidal activity of CTT against these bacterial strains.
Main Methods:
- Scanning electron microscopy was employed to visualize morphological alterations.
- Bacterial strains, Escherichia coli NY-17 and Serratia marcescens IID 620, were exposed to varying concentrations of CTT and CMZ.
Main Results:
- Cefotetan induced filamentous forms in E. coli at 0.024 µg/ml, while cefmetazole required 0.2 µg/ml for similar effects.
- Both strains exhibited spheroplast-like structures and cell lysis at lower concentrations with CTT compared to CMZ.
- Cefotetan displayed bactericidal activity against both E. coli and S. marcescens.
Conclusions:
- Cefotetan exhibits significantly greater potency than cefmetazole in causing morphological damage and bacterial death.
- CTT is a more effective bactericidal agent against the tested strains of E. coli and S. marcescens.