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Related Experiment Videos

Function of granulocytes collected for transfusion

R G Strauss

    Progress in Clinical and Biological Research
    |January 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Granulocyte transfusions prepared by centrifugation leukapheresis show normal in vitro function. However, filtration leukapheresis impairs neutrophil function in vivo, making centrifugation the preferred method.

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    Area of Science:

    • Hematology
    • Transfusion Medicine
    • Cellular Therapy

    Background:

    • Granulocyte transfusions are used to treat severe neutropenia.
    • Various leukapheresis techniques exist for collecting granulocytes.
    • Understanding the functional integrity of collected granulocytes is crucial for transfusion efficacy.

    Purpose of the Study:

    • To review the in vitro properties of granulocytes prepared by different leukapheresis methods.
    • To compare the functional performance of neutrophils collected via centrifugation versus filtration leukapheresis.

    Main Methods:

    • Literature review of in vitro studies on granulocytes.
    • Analysis of functional assays comparing centrifugation and filtration leukapheresis.

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    Main Results:

    • Neutrophils from centrifugation (continuous-flow, intermittent-flow, gravity) and filtration leukapheresis exhibit normal structure, biochemistry, and in vitro microbicidal activity.
    • While cell movement appears satisfactory in vitro, impaired circulatory kinetics and in vivo migration are suspected for centrifugation-collected cells.
    • Filtration leukapheresis-collected neutrophils demonstrate inferior in vivo functional performance compared to centrifugation-collected cells.

    Conclusions:

    • Centrifugation leukapheresis yields granulocytes with acceptable in vitro properties.
    • Filtration leukapheresis significantly impairs neutrophil function in vivo.
    • Currently, granulocyte units prepared by centrifugation leukapheresis are recommended for transfusion.