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Anomalous patterns in cultured cell monolayers

E M Adler, L J Flunk, J M Mullin

    Science (New York, N.Y.)
    |August 27, 1982
    PubMed
    Summary
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    Cell monolayers unexpectedly form grid patterns due to temperature fluctuations affecting cell attachment, not growth. This widespread phenomenon in cell culture requires further investigation into adhesion mechanisms.

    Area of Science:

    • Cell Biology
    • Biophysics

    Background:

    • Cell monolayers are fundamental in biological research.
    • Consistent cell distribution is typically assumed in standard cell culture techniques.

    Purpose of the Study:

    • To investigate the formation of gridlike patterns in evenly seeded cell monolayers.
    • To identify the underlying mechanisms responsible for these observed patterns.

    Main Methods:

    • Observation of cell density patterns in multiple cell lines.
    • Analysis of environmental factors within cell incubators.
    • Evaluation of the time course of pattern development.

    Main Results:

    • Gridlike patterns of varying cell density were observed in five out of six tested cell lines.

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  • Pattern formation was linked to small, transient temperature changes associated with incubator tray structure.
  • The rapid appearance of patterns suggests attachment, not growth, is the primary affected factor.

    • Conclusions:

    • The formation of gridlike patterns in cell monolayers is a widespread phenomenon.
    • Thermal microcurrents induced by incubator trays likely impair cell adhesion or cause directed sedimentation.
    • Further research is needed to elucidate the precise biophysical mechanisms involved.