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Cell lines from sheep and cattle blood

Z Woldehiwet, R H Johnson, G R Scott

Veterinary Research Communications

|December 1, 1981

Summary

Establishing ovine and bovine cell lines from blood is simplified using a reproducible method. This technique involves overnight adherence of buffy coat cells, followed by culture with various serum supplements for successful cell line development.

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Area of Science:

Veterinary Science
Cell Biology
Animal Biotechnology

Background:

Establishing stable cell lines from livestock blood is crucial for research and diagnostics.
Existing methods can be complex and time-consuming, requiring optimization for different species.

Purpose of the Study:

To develop a simple and reproducible method for establishing ovine (sheep) and bovine (cattle) cell lines from blood.
To evaluate the efficacy of different serum supplements in cell line establishment.

Main Methods:

Buffy coat cells from sheep and cattle were isolated.
Cells were allowed to adhere to culture flasks in media with autologous plasma overnight.
Following adherence, cells were cultured in growth medium supplemented with either non-inactivated foetal calf serum, lamb serum, or autologous serum.

Main Results:

Ovine cell lines were successfully established using all tested serum supplements.
Bovine cell lines demonstrated more efficient establishment when unheated autologous serum was used as a supplement.
The method proved to be simple and reproducible for both species.

Conclusions:

A straightforward and reproducible protocol for establishing ovine and bovine cell lines from blood has been established.
Autologous serum is a key factor for efficient bovine cell line establishment, while ovine cell lines show flexibility in serum requirements.
This method provides a valuable tool for veterinary research and diagnostics.

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