Detection, identification, and partial characterization of serine proteases and esterases in biological systems

The Limulus Blood Cell Secretes α2-Macroglobulin When Activated.

The Biological bulletin·2018

In vivo cleaved CDCP1 promotes early tumor dissemination via complexing with activated β1 integrin and induction of FAK/PI3K/Akt motility signaling.

Oncogene·2012

Blocking of CDCP1 cleavage in vivo prevents Akt-dependent survival and inhibits metastatic colonization through PARP1-mediated apoptosis of cancer cells.

Oncogene·2011

Mutation analysis of membrane type-1 matrix metalloproteinase (MT1-MMP). The role of the cytoplasmic tail Cys(574), the active site Glu(240), and furin cleavage motifs in oligomerization, processing, and self-proteolysis of MT1-MMP expressed in breast carcinoma cells.

The Journal of biological chemistry·2001

Growth factor-induced angiogenesis in vivo requires specific cleavage of fibrillar type I collagen.

Blood·2001

The isolation, characterization, and molecular cloning of a 75-kDa gelatinase B-like enzyme, a member of the matrix metalloproteinase (MMP) family. An avian enzyme that is MMP-9-like in its cell expression pattern but diverges from mammalian gelatinase B in sequence and biochemical properties.

The Journal of biological chemistry·2000

Identification of MDV and REV Using RPA-CRISPR/Cas12a Combined with Fluorescence and Lateral Flow Assays.

Analytical biochemistry·2026

Selective fluorescence turn-on detection of methylglyoxal using N-substituted NBD derivatives.

Analytical biochemistry·2026

Simultaneous GC-MS Determination of Unsubstituted, Hydroxy-, Amino-, and Hydroxy-amino Short-Chain Fatty Acids Together with Amino Acids in Biological and Food Samples Following Derivatization.

Analytical biochemistry·2026

Validation of SARS-CoV-2 neutralization assay using VSV-based pseudovirus system.

Analytical biochemistry·2026

Development and Validation of a Mechanism-Based Ba/F3-hGHR Bioassay for the Potency Assessment of Recombinant Human Growth Hormone.

Analytical biochemistry·2026

Camel milk-derived extracellular vesicles using a two-step PEG precipitation strategy.

Analytical biochemistry·2026

Assays of Thiol Isomerase Activity.

Methods in molecular biology (Clifton, N.J.)·2026

Nanopore-enabled time-resolved monitoring of catecholamine-related phenylalanine metabolism.

Nature nanotechnology·2026

Heme handling in the system II heme lyase CcsBA from Bacteroides thetaiotaomicron.

Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry·2026

Quantifying the Characteristic Turnover Frequency of an Individual Redox Enzyme via Real-Time Electrical Transduction of Catalytic Events in a Label-Free Single-Protein Junction.

Journal of the American Chemical Society·2026

RNase E resolves toxic condensates by counteracting phase separation of Type II RhlB helicases.

Nucleic acids research·2026

Assessment of Bacterial N-Degron Proteolysis by a Dual-Fluorescence Reporter.

Methods in molecular biology (Clifton, N.J.)·2026

Related Experiment Videos

Detection, identification, and partial characterization of serine proteases and esterases in biological systems

Related Concept Videos

The Limulus Blood Cell Secretes α2-Macroglobulin When Activated.

In vivo cleaved CDCP1 promotes early tumor dissemination via complexing with activated β1 integrin and induction of FAK/PI3K/Akt motility signaling.

Blocking of CDCP1 cleavage in vivo prevents Akt-dependent survival and inhibits metastatic colonization through PARP1-mediated apoptosis of cancer cells.

Mutation analysis of membrane type-1 matrix metalloproteinase (MT1-MMP). The role of the cytoplasmic tail Cys(574), the active site Glu(240), and furin cleavage motifs in oligomerization, processing, and self-proteolysis of MT1-MMP expressed in breast carcinoma cells.

Growth factor-induced angiogenesis in vivo requires specific cleavage of fibrillar type I collagen.

The isolation, characterization, and molecular cloning of a 75-kDa gelatinase B-like enzyme, a member of the matrix metalloproteinase (MMP) family. An avian enzyme that is MMP-9-like in its cell expression pattern but diverges from mammalian gelatinase B in sequence and biochemical properties.

Identification of MDV and REV Using RPA-CRISPR/Cas12a Combined with Fluorescence and Lateral Flow Assays.

Selective fluorescence turn-on detection of methylglyoxal using N-substituted NBD derivatives.

Simultaneous GC-MS Determination of Unsubstituted, Hydroxy-, Amino-, and Hydroxy-amino Short-Chain Fatty Acids Together with Amino Acids in Biological and Food Samples Following Derivatization.

Validation of SARS-CoV-2 neutralization assay using VSV-based pseudovirus system.

Development and Validation of a Mechanism-Based Ba/F3-hGHR Bioassay for the Potency Assessment of Recombinant Human Growth Hormone.

Camel milk-derived extracellular vesicles using a two-step PEG precipitation strategy.

Assays of Thiol Isomerase Activity.

Nanopore-enabled time-resolved monitoring of catecholamine-related phenylalanine metabolism.

Heme handling in the system II heme lyase CcsBA from Bacteroides thetaiotaomicron.

Quantifying the Characteristic Turnover Frequency of an Individual Redox Enzyme via Real-Time Electrical Transduction of Catalytic Events in a Label-Free Single-Protein Junction.

RNase E resolves toxic condensates by counteracting phase separation of Type II RhlB helicases.

Assessment of Bacterial N-Degron Proteolysis by a Dual-Fluorescence Reporter.

Related Experiment Videos

Detection, identification, and partial characterization of serine proteases and esterases in biological systems

Related Concept Videos

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