A method for the intraocular injection of micro-volumes in small animal species

A method has been developed for the intraocular administration of small volumes (0.5-1.5 microliters) in small animal species such as guniea pigs and rats. The method was found to yield reproducible injection volumes and resulted in minimal ocular trauma. The aqueous humor of eyes injected with saline contained normal levels of protein and was free of white blood cells. Responses to Prostaglandin E2 and E.Coli endotoxin were dose-dependent and as expected.