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Related Experiment Videos

Pleiotropic control of five eucaryotic genes by multiple regulatory elements

V Turoscy, T G Cooper

    Journal of Bacteriology
    |September 1, 1982
    PubMed
    Summary

    Mutants unable to induce allantoin degradation enzymes in Saccharomyces cerevisiae were identified. These mutants show a defect in enzyme induction, not nitrogen catabolite repression, indicating specific regulatory roles.

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    Area of Science:

    • Microbiology
    • Molecular Biology
    • Biochemistry

    Background:

    • Allophanate is an inducer for five structural genes involved in allantoin degradation in Saccharomyces cerevisiae.
    • This suggests a common regulatory mechanism for these genes.

    Purpose of the Study:

    • To test the hypothesis of common control of allantoin degradation genes.
    • To identify and characterize mutants defective in the induction of these enzymes.

    Main Methods:

    • Screening for mutants unable to induce allantoin-degrading enzymes.
    • Phenotypic analysis of mutant growth on allantoin pathway intermediates and other nitrogen sources.
    • Complementation tests to determine allelism with known allantoin pathway loci.
    • Analysis of enzyme levels in wild-type and mutant strains under different growth conditions.

    Main Results:

    • Mutants (dal81) were identified that grew poorly on allantoin pathway intermediates but utilized other nitrogen sources normally.
    • These mutations were recessive and complemented known allantoin pathway loci.
    • The dal81 locus was not tightly linked to allantoin pathway structural genes.
    • Dal81- strains exhibited low basal enzyme levels, unaffected by the inducer, but showed moderate increases on poor nitrogen sources.

    Conclusions:

    • Dal81 mutant strains have a defect in enzyme induction, not in nitrogen catabolite repression.
    • The products of DAL80 and DAL81 loci may function with considerable independence.

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