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Rapid analysis of immunoglobulin isoelectric focusing patterns with cellulose nitrate sheets and immunoperoxidase
Immunoglobulins which have been focused to their isoelectric points in thin-layer polyacrylamide gels can be transferred by diffusion to the surface of cellulose nitrate sheets with retention of the original focusing patterns. IgG can be detected with peroxidase-linked antiserum. Unlike polyacrylamide gel, cellulose nitrate offers no permeability barrier, and so washing times are reduced from days to minutes. Thus small quantities (less than 1 microgram) of IgG may be visualized within 24 h of isoelectric focusing. In addition after immobilization on cellulose nitrate, immunoglobulin reactivity with antigens, including those of high molecular weight, may be analysed.
Immunoglobulins which have been focused to their isoelectric points in thin-layer polyacrylamide gels can be transferred by diffusion to the surface of cellulose nitrate sheets with retention of the original focusing patterns. IgG can be detected with peroxidase-linked antiserum. Unlike polyacrylamide gel, cellulose nitrate offers no permeability barrier, and so washing times are reduced from days to minutes. Thus small quantities (less than 1 microgram) of IgG may be visualized within 24 h of isoelectric focusing. In addition after immobilization on cellulose nitrate, immunoglobulin reactivity with antigens, including those of high molecular weight, may be analysed.