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Electrical development in spinal cord cell culture

M B Jackson, H Lecar, D E Brenneman

    The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
    |August 1, 1982
    PubMed
    Summary
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    Spontaneous electrical activity in mouse spinal cord cultures increases rapidly, correlating with developmental changes. This suggests ongoing neuronal activity is crucial for development and neurotransmitter system maturation.

    Area of Science:

    • Neuroscience
    • Developmental Biology
    • Cellular Electrophysiology

    Background:

    • Neuronal development involves complex electrophysiological and neurochemical changes.
    • Understanding the role of spontaneous electrical activity in neuronal maturation is critical.

    Purpose of the Study:

    • To investigate the correlation between spontaneous electrical activity and neurochemical development in mouse spinal cord cultures.
    • To examine the developmental timelines of excitatory and inhibitory synaptic activity.
    • To compare the effects of GABA and glycine on neuronal activity during development.

    Main Methods:

    • Parallel electrophysiological recordings using extracellular patch electrodes and neurochemical assays.
    • Assessment of choline acetyltransferase activity in response to tetrodotoxin.

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  • Application of gamma-aminobutyric acid (GABA) and glycine to evaluate their effects on spontaneous activity.

    • Main Results:

    • Spontaneous electrical activity increased significantly within the first 2 weeks of culture.
    • Tetrodotoxin-induced depression of choline acetyltransferase activity correlated with increased spontaneous activity.
    • Excitatory synaptic activity and action potentials developed concurrently, peaking by week 2.
    • Glycine's effectiveness in suppressing activity increased with culture age, paralleling inhibitory postsynaptic potential development.

    Conclusions:

    • Ongoing electrical activity plays a significant role in neuronal development.
    • Distinct developmental timetables exist for GABA and glycine receptor systems.
    • Glycine receptor development appears to be closely linked to the maturation of inhibitory synaptic function.