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Human growth hormone: 1974-1981

C H Li

    Molecular and Cellular Biochemistry
    |July 7, 1982
    PubMed
    Summary

    This review covers chemical studies of human growth hormone (HGH) from 1974-1981. Research explored HGH modifications, fragments, and synthesis for a better understanding of its structure and function.

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    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Endocrinology

    Background:

    • Human growth hormone (HGH) is a crucial protein hormone regulating growth and metabolism.
    • Understanding HGH's chemical structure is vital for its therapeutic applications and biological function.
    • Previous research (1974-1981) laid the groundwork for HGH's chemical characterization.

    Purpose of the Study:

    • To review chemical investigations of the human growth hormone (HGH) molecule.
    • To summarize key findings in HGH chemical modification and fragmentation studies.
    • To highlight advancements in synthetic and recombinant HGH research.

    Main Methods:

    • Chemical modification of HGH.
    • Selective enzymic cleavage of HGH.
    • Synthesis and complementation of HGH peptide fragments.
    • Covalent reconstitution of HGH fragments.
    • Bacterial synthesis of methionyl-HGH.

    Main Results:

    • Characterization of the 20K HGH variant.
    • Successful synthesis of biologically active HGH peptide fragments.
    • Demonstration of fragment complementation to form functional HGH.
    • Development of methods for HGH reconstitution and bacterial production.

    Conclusions:

    • Chemical studies significantly advanced the understanding of HGH structure-function relationships.
    • Fragment-based approaches provided insights into HGH's active sites.
    • Synthetic and recombinant HGH methods paved the way for therapeutic advancements.

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