Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An in vivo model for studying platelet aggregation and disaggregation

C P Page, W Paul, J Morley

    Thrombosis and Haemostasis
    |June 28, 1982
    PubMed
    Summary

    Researchers developed a new, minimally invasive method to track how blood cells called platelets clump together and break apart inside living animals. By labeling platelets with a radioactive tracer, the team could monitor their movement between the chest and abdomen. They tested various substances that either trigger or prevent clumping, proving the method is reliable for future drug testing.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Synergistic Interactions Between the Quinolone-Derived Compound HT61 and Tobramycin Against a Range of Clinical Isolates of Pseudomonas aeruginosa In Vitro.

    Lung·2025
    Same author

    A novel murine model of pulmonary fibrosis: the role of platelets in chronic changes induced by bleomycin.

    Journal of pharmacological and toxicological methods·2021
    Same author

    The small quinolone derived compound HT61 enhances the effect of tobramycin against Pseudomonas aeruginosa in vitro and in vivo.

    Pulmonary pharmacology & therapeutics·2019
    Same author

    Pharmacology and Therapeutics of Bronchodilators Revisited.

    Pharmacological reviews·2019
    Same author

    Radiolabelling and immunohistochemistry reveal platelet recruitment into lungs and platelet migration into airspaces following LPS inhalation in mice.

    Journal of pharmacological and toxicological methods·2019
    Same author

    Use of animal models in IPF research.

    Pulmonary pharmacology & therapeutics·2018

    Area of Science:

    • Hematology research within cardiovascular medicine
    • In vivo platelet aggregation monitoring techniques

    Background:

    No prior work had resolved how to continuously observe blood cell behavior within living subjects without highly invasive surgery. That uncertainty drove the need for a non-destructive tracking approach. It was already known that traditional laboratory tests often fail to replicate complex physiological environments. Prior research has shown that isolated cell samples lack the influence of surrounding tissues and blood flow dynamics. This gap motivated the creation of a system capable of monitoring labeled cells in real time. Scientists previously lacked a reliable way to quantify clumping events across different body regions simultaneously. That limitation hindered the assessment of potential therapeutic compounds in a natural setting. This study addresses these challenges by introducing a refined tracking protocol for experimental models.

    Purpose Of The Study:

    The study aims to establish a simple, minimally invasive technique for the continuous monitoring of platelet behavior in living subjects. Researchers sought to overcome the limitations of existing methods that often require invasive surgical procedures. The team focused on tracking 111-Indium labelled homologous platelets to observe their movement between the thoracic and abdominal regions. This investigation was motivated by the need for a reliable system to study clumping and breaking apart events in real time. By testing various aggregatory and anti-aggregatory agents, the authors intended to validate the sensitivity of their new approach. They specifically examined the effects of ADP, collagen, and platelet activating factor on cell distribution. The researchers also aimed to demonstrate the utility of the method in the presence of minimal heparin. This work provides a foundation for more accurate assessments of therapeutic agents within a physiological environment.

    Keywords:
    111-Indium labelingcardiovascular pharmacologyguinea-pig modelheparinized blood

    Frequently Asked Questions

    The researchers propose that aggregatory agents like ADP, collagen, and PAF trigger a shift in labeled platelets from the abdominal to the thoracic region. This movement increases the thoracic-to-abdominal count ratio, whereas the anti-aggregatory agent PGI2 effectively dampens this specific response.

    The team utilizes 111-Indium as a radioactive tracer to label homologous platelets. This specific isotope allows for the continuous monitoring of cell movement between the thoracic and abdominal regions throughout the experimental duration.

    The authors state that the technique remains functional in the presence of minimal heparin. This technical necessity ensures that the blood remains fluid enough for accurate monitoring while minimizing the potential interference of anticoagulants with natural cell behavior.

    Related Experiment Videos

    Main Methods:

    The investigators employed a minimally invasive design to track radioactive tracers within guinea-pigs. They utilized 111-Indium to tag homologous cells for continuous observation across two distinct anatomical zones. The review approach involved administering specific chemical triggers to induce clumping responses. Researchers monitored the thoracic and abdominal regions to calculate the relative distribution of these tagged cells. They tested the influence of various substances, including collagen and prostacyclin, on the observed cell movement. The team maintained consistent experimental conditions to ensure the reliability of the collected data. They performed these assessments in the presence of heparin to prevent premature clotting during the procedure. This systematic protocol allowed for the precise quantification of physiological changes over time.

    Main Results:

    Key findings from the literature demonstrate that the administration of ADP, collagen, or PAF consistently increases counts in the thoracic region. Simultaneously, these agents cause a measurable decrease in counts within the abdominal area. This shift results in a significant rise in the thoracic-to-abdominal ratio. The team observed that the increase in this ratio is notably more protracted following collagen administration than after ADP or PAF exposure. Furthermore, the application of 50-500 ng/kg of prostacyclin effectively reduces the response to ADP. The data confirm that the technique is both simple and highly reproducible. These results highlight the sensitivity of the model to both aggregatory and anti-aggregatory stimuli. The findings establish a clear correlation between agent type and the resulting physiological response pattern.

    Conclusions:

    The authors propose that their tracking system offers a robust platform for evaluating novel anti-aggregatory compounds. Synthesis and implications suggest that this model effectively captures the dynamic nature of blood cell behavior in living subjects. The researchers claim the technique remains functional even when heparin levels are kept to a minimum. Findings indicate that the observed responses to various agents are consistent and reproducible across different trials. The team notes that the protracted response to collagen compared to other agents highlights distinct temporal patterns in clumping. They suggest that the observed reduction in response following prostacyclin treatment validates the utility of this approach. The study provides a framework for future investigations into the mechanisms governing blood cell interactions. These results support the use of this model for assessing drug efficacy in a physiological context.

    The researchers rely on the thoracic-to-abdominal count ratio as the primary data type. This measurement serves as a proxy for assessing the degree of cell clumping, with higher values indicating increased aggregation in the chest area.

    The team measures the duration of the response following the administration of different agents. They observe that collagen induces a more protracted rise in the thoracic-to-abdominal ratio compared to the shorter, more transient effects produced by ADP or PAF.

    The researchers propose that this model is highly suitable for the evaluation of anti-aggregatory agents in vivo. They suggest that the simplicity and reproducibility of the method make it a valuable tool for future pharmacological studies.