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Problems in the measurement of putative serum immune complexes by the method of Beaumont

J W Goldzieher, N D Greene, M C Williams

    American Journal of Reproductive Immunology : AJRI : Official Journal of the American Society for the Immunology of Reproduction and the International Coordination Committee for Immunology of Reproduction
    |June 1, 1982
    PubMed

    A methodological investigation of the procedure used by Beaumont et al for measuring a "serum immune complex" precipitated by 25% ammonium sulfate and alleged to contain an ethynyl-estradiol binding immunoglobulin has found major problems with reproducibility and with the correlation of total protein as measured by the Lowry method and the IgG content as determined by a specific nephelometric procedure.

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