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Determination of thiosulphate in urine
Scandinavian Journal of Clinical and Laboratory Investigation
|October 1, 1978
Summary
This study details a new method for measuring thiosulphate in urine. Healthy individuals excrete approximately 31.7 micromoles of thiosulphate per 24 hours.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Clinical Chemistry
Background:
- Thiosulphate is an endogenous compound with potential roles in biological systems.
- Accurate quantification of thiosulphate in biological matrices like urine is crucial for metabolic studies.
- Existing methods for thiosulphate determination may be limited by interference or complexity.
Purpose of the Study:
- To develop and validate a novel colorimetric method for the precise determination of thiosulphate in human urine.
- To establish reference excretion levels of thiosulphate in healthy human subjects.
Main Methods:
- Urine samples were treated to remove interfering compounds, including thiocyanate, using ion exchange chromatography.
- Thiosulphate was chemically converted to thiocyanate using cyanide and cupric ions.
- Thiocyanate was concentrated via ion exchange, eluted with ferric ions, and quantified colorimetrically.
Main Results:
- The developed method effectively removes interfering substances and quantifies thiosulphate.
- Healthy human subjects demonstrated a mean thiosulphate excretion of 31.7 ± 12.8 μmol/24 h.
- The method provides a reliable means for assessing thiosulphate levels in urine.
Conclusions:
- A robust and accurate colorimetric assay for urinary thiosulphate has been established.
- The findings provide baseline data on thiosulphate excretion in healthy populations.
- This method can be valuable for future research into thiosulphate metabolism and its clinical significance.