Evidence that rat peripheral myelin does not contain the rat spinal cord protein (RSCP-PN)

Rat spinal cord protein (SCP) from peripheral nerves (RSCP-PN) was not detected in purified rat peripheral nerve myelin by sodium dodecyl sulfate (SDS)-polyacrylamide slab gel electrophoresis or by immunodiffusion analyses using an anti-rat SCP in peripheral nerve (RSCP-PN) serum. The slab gel electrophoretic analyses also revealed that RSCP-PN has an appreciably lower molecular size than the component of rat peripheral myelin that is identified as P2 by its molecular size of 13,600 daltons. Thus, RSCP-PN and rat P2 are unrelated proteins.