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Surface properties of Chlamydia psittaci

D W Vance, T P Hatch

Infection and Immunity

|July 1, 1980

Summary

Chlamydia psittaci elementary bodies are hydrophobic and negatively charged at neutral pH. Surface charge modifications affect C. psittaci aggregation, potentially influencing host cell interactions.

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Area of Science:

Microbiology
Cell Biology
Biophysics

Background:

Chlamydia psittaci is an obligate intracellular bacterium.
Understanding the surface properties of elementary bodies is crucial for elucidating host-pathogen interactions.

Purpose of the Study:

To investigate the surface characteristics of Chlamydia psittaci elementary bodies.
To determine the role of surface hydrophobicity and charge in C. psittaci behavior.

Main Methods:

Diethylaminoethyl-Sephadex chromatography
Cytophoresis
Aqueous polymer two-phase system partitioning
Hydrophobic interaction chromatography

Main Results:

The surface of C. psittaci was found to be hydrophobic.
The organism exhibited a negative surface charge at pH 7.
The isoelectric point was determined to be approximately pH 5.
Blocking carboxyl groups altered surface charge and induced agglomeration.

Conclusions:

Surface hydrophobicity and negative charge are key properties of C. psittaci.
Surface charge modifications influence C. psittaci aggregation.
These surface properties may play a significant role in the phagocytosis of C. psittaci by host cells.

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