Cytogenetic effects of Rhodesian chrysotile on human lymphocytes in vitro

Cytogenetic studies were carried out on human lymphocytes from nine subjects; cells were incubated with Rhodesian chrysotile fibres (10 micrograms/ml) for 48 and 72 hours. After treatment, chromosome alterations were twice as frequent, and chromosome breaks showed the highest percentage increment. We focussed our attention on chromosome breaks, since they are typically induced by ionizing radiation and by chemical carcinogens, and in particular alkylating agents. Since asbestos strongly adsorbs proteins and since indications exist that the interaction may be selective, we consider that this type of interaction may be the cause of cytogenetic damage induced in vitro by asbestos.