Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Heparin with two binding sites for antithrombin or platelet factor 4

R E Jordan, L V Favreau, E H Braswell

    The Journal of Biological Chemistry
    |January 10, 1982
    PubMed
    Summary

    A novel affinity chromatography method separates heparin by molecular size and anticoagulant activity. Highly active heparin fractions show distinct antithrombin binding properties and potent anticoagulant effects.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Living with COPD and its psychological effects on participating in community-based physical activity in Brazil: a qualitative study. Findings from the Breathe Well group.

    NPJ primary care respiratory medicine·2024
    Same author

    Accuracy and economic evaluation of screening tests for undiagnosed COPD among hypertensive individuals in Brazil.

    NPJ primary care respiratory medicine·2022
    Same author

    Accuracy of Vitalograph lung monitor as a screening test for COPD in primary care.

    NPJ primary care respiratory medicine·2020
    Same author

    Systematic review of the effectiveness of community-based self-management interventions among primary care COPD patients.

    NPJ primary care respiratory medicine·2018
    Same author

    Systematic review: chronic obstructive pulmonary disease and work-related outcomes.

    Occupational medicine (Oxford, England)·2018
    Same author

    Cohort Profile: The Birmingham Chronic Obstructive Pulmonary Disease (COPD) Cohort Study.

    International journal of epidemiology·2016

    Area of Science:

    • Biochemistry
    • Chromatography
    • Pharmacology

    Background:

    • Heparin is a complex mucopolysaccharide with varying molecular sizes and anticoagulant potencies.
    • Fractionating heparin is crucial for understanding its biological activity and therapeutic applications.

    Purpose of the Study:

    • To develop a highly discriminating affinity chromatographic technique for heparin fractionation.
    • To characterize different heparin species based on their interactions with antithrombin and anticoagulant activity.

    Main Methods:

    • Affinity chromatography using antithrombin and concanavalin A-Sepharose.
    • Analytical ultracentrifugation to determine molecular weight and heterogeneity.
    • Fluorescence spectroscopy to study protein-heparin interactions and binding kinetics.

    Related Experiment Videos

    Main Results:

    • A new method effectively fractionated heparin species across all molecular sizes.
    • Highly active heparin fractions (LMW and HMW) exhibited distinct antithrombin affinities and high anticoagulant potencies (350 and 731 units/mg).
    • Stoichiometry indicated 2 protein molecules bind to 1 heparin molecule, with specific binding constants for antithrombin and greater avidity for platelet factor 4.

    Conclusions:

    • The developed chromatographic technique provides a powerful tool for heparin characterization.
    • Highly active heparin fractions possess specific binding characteristics with antithrombin and other proteins.
    • Understanding these interactions is key to optimizing heparin-based therapeutics.