Effects of lipid peroxidation on prostaglandin synthesis in rabbit kidney medulla slices

Ascorbic acid and Fe2+ had a more powerful stimulatory effect on the lipid peroxidation of rabbit kidney medullar slices in 0.15 M KCl/0.02 M Tris-HCl buffer than in Krebs-Henseleit buffer. The lipid peroxidation induced by ascorbic acid and Fe2+ enhanced the release of unsaturated fatty acids from tissue lipids, but inhibited medullary generation of prostaglandin E. These results suggest that the lipid peroxidation induced by ascorbic acid and Fe2+ may inhibit the formation of prostaglandin E and that this inhibitory effect may be mediated by lipid peroxides via the inhibition of prostaglandin synthetase.