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Related Experiment Videos

Estrogen receptors in the turtle brain

P Mak, S M Ho, I P Callard

    Brain Research
    |January 7, 1982
    PubMed
    Summary

    Researchers found estrogen binding components (EBC) in the freshwater turtle brain, similar to estrogen receptors. These receptors show high affinity and specificity, with concentrations varying seasonally and highest in the hypothalamus-preoptic areas.

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    Area of Science:

    • Neuroendocrinology
    • Comparative Endocrinology
    • Molecular Endocrinology

    Background:

    • Estrogen receptors play crucial roles in the central nervous system (CNS).
    • Understanding estrogen binding in non-mammalian vertebrates provides insights into receptor evolution and function.
    • The freshwater turtle, Chrysemys picta, serves as a model for studying reptilian neurobiology.

    Purpose of the Study:

    • To investigate the presence and characteristics of estrogen binding activity in the CNS of Chrysemys picta.
    • To determine the binding affinity, capacity, and specificity of the estrogen binding component (EBC).
    • To map the distribution of EBC within the turtle brain and assess seasonal variations.

    Main Methods:

    • DNA-cellulose affinity chromatography was employed to isolate and characterize EBC from brain cytosol extracts.
    • Binding assays were performed to determine affinity (Kd) and capacity (n).
    • Specificity was assessed using various steroid hormones, and tissue distribution was analyzed.

    Main Results:

    • An estrogen binding component (EBC) with characteristics of an estrogen receptor was identified in both male and female turtle brain cytosol.
    • The EBC demonstrated high affinity (Kd = 10^-10 M) and specificity for estrogens, excluding progesterone and androgens.
    • EBC was highest in the hypothalamus-preoptic areas (HPOA) and remaining forebrain (RFB), with lower levels in the mid/hindbrain (HB).
    • Specific estradiol-17 beta binding was absent in plasma and non-target tissues (lung, kidney, muscle).
    • Seasonal variations in EBC concentrations were observed between May and October.

    Conclusions:

    • A functional estrogen receptor-like molecule is present in the CNS of Chrysemys picta.
    • The localization and specificity suggest a significant role for estrogen in regulating CNS functions in this species.
    • Seasonal fluctuations in EBC indicate potential hormonal influences on neurobiological processes throughout the year.

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