Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A candidate reference method for uric acid in serum. II. Interlaboratory testing

P Duncan, N Gochman, D Bayse

    Clinical Chemistry
    |February 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Implementation of non-medical prescribing by therapeutic radiographers: Views of prescribers and service managers in devolved UK health systems.

    Radiography (London, England : 1995)·2026
    Same author

    Social Deprivation among Children with Inherited Metabolic Disorders.

    Irish medical journal·2025
    Same author

    Relevance of capsule endoscopy in patients with obscure gastrointestinal bleeding - a comprehensive real-world study.

    Journal of physiology and pharmacology : an official journal of the Polish Physiological Society·2025
    Same author

    Therapeutic radiographer prescribing practices in the United Kingdom: Questionnaire survey.

    Radiography (London, England : 1995)·2024
    Same author

    Development of a model of medication review for use in clinical practice: Bristol medication review model.

    BMC medicine·2021
    Same author

    Collaboration between the military and a charity: delivering care to patients, training to defence medical staff and defence engagement overseas.

    BMJ military health·2021
    Same journal

    Comparison of Information-Dependent Acquisition and Sequential Window Acquisition of All Theoretical Mass Spectra for Untargeted Drug Testing on a Linear Ion Trap-Pulsing Quadrupole-Time of Flight Mass Spectrometer.

    Clinical chemistry·2026
    Same journal

    Patterns of One-Year Change in HbA1c and Continuous Glucose Monitoring (CGM) Metrics in Older Adults with Type 2 Diabetes.

    Clinical chemistry·2026
    Same journal

    TSH Pediatric Reference Intervals: Lack of CALIPER Applicability to US-Based Populations.

    Clinical chemistry·2026
    Same journal

    Rapid Detection of Hemoglobinopathy Variants Using One-Step Library Preparation and Nanopore Sequencing.

    Clinical chemistry·2026
    Same journal

    Editor's Note: Circulating Proteolytic Products of Carboxypeptidase N for Early Detection of Breast Cancer.

    Clinical chemistry·2026
    Same journal

    In Reply to Reflexing NT-proBNP for sFlt-1/PlGF Ratios That Fall into the Measurement Uncertainty for Preeclampsia Risk Classification.

    Clinical chemistry·2026
    See all related articles

    A new reference method for measuring uric acid in serum was tested across 12 laboratories. This ultrasound spectrophotometric method demonstrated good precision and reliability for clinical diagnostics.

    Area of Science:

    • Clinical Chemistry
    • Analytical Chemistry
    • Biochemical Assays

    Background:

    • Accurate measurement of uric acid in serum is crucial for diagnosing conditions like gout and kidney disease.
    • Existing methods may lack standardization or sufficient precision for reliable clinical interpretation.
    • A validated reference method is needed to ensure consistency in uric acid quantitation across different laboratories.

    Purpose of the Study:

    • To evaluate the performance of a candidate Reference Method for serum uric acid determination.
    • To assess the transferability, precision, and reliability of the proposed method through interlaboratory testing.
    • To establish a standardized approach for accurate uric acid quantitation in clinical samples.

    Main Methods:

    • The candidate Reference Method utilizes ultrasound spectrophotometry for uric acid quantitation.

    Related Experiment Videos

  • Measurements are taken before and after enzymatic incubation with uricase.
  • Interlaboratory testing involved 12 independent laboratories to assess method reproducibility.

    • Main Results:

    • The interlaboratory coefficient of variation (CV) ranged from 2% to 6% for serum uric acid concentrations between 0.12 and 0.60 mmol/L.
    • The method demonstrated high precision and accuracy across participating laboratories.
    • Successful duplication of results among different laboratory settings was confirmed.

    Conclusions:

    • The candidate Reference Method for serum uric acid is transferable and reliable.
    • The method exhibits satisfactory intra- and interlaboratory precision for clinical use.
    • This validated reference method provides a standardized approach for accurate uric acid measurement in serum.