Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Development of solid-phase immunoassay using chemiluminescent IgG conjugates

P J Cheng, I Hemmilä, T Lövgren

    Journal of Immunological Methods
    |January 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    LOAd703-induced tumor microenvironment gene engineering in combination with atezolizumab in metastatic malignant melanoma: a phase I/II trial.

    Nature communications·2026
    Same author

    The impact of a novel transendometrial approach for caesarean myomectomy on obstetric outcomes of subsequent pregnancy: a longitudinal panel study.

    BJOG : an international journal of obstetrics and gynaecology·2017
    Same author

    China's new two-child policy: maternity care in the new multiparous era.

    BJOG : an international journal of obstetrics and gynaecology·2016
    Same author

    Genome-wide normalized score: a novel algorithm to detect fetal trisomy 21 during non-invasive prenatal testing.

    Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2014
    Same author

    Effect of C-H bonds on the quenching of luminescent lanthanide chelates.

    Journal of fluorescence·2013
    Same author

    Synthetic single-framework antibody library integrated with rapid affinity maturation by VL shuffling.

    Protein engineering, design & selection : PEDS·2011

    This study explored solid-phase luminescent immunoassay (LIA) using novel conjugates. The luminescent immunoassay (LIA) system offers a stable, cost-effective, and sensitive alternative to radioimmunoassay (RIA).

    Area of Science:

    • Biochemistry
    • Immunology
    • Analytical Chemistry

    Background:

    • Solid-phase luminescent immunoassay (LIA) is an emerging diagnostic technique.
    • Aminobutyl-ethylisoluminol-IgG conjugates are key reagents in LIA development.

    Purpose of the Study:

    • To investigate the efficacy of solid-phase luminescent immunoassay (LIA) using aminobutyl-ethylisoluminol-IgG conjugates.
    • To evaluate different solid-phase supports for optimal assay performance.

    Main Methods:

    • Conjugation of aminobutyl-ethylisoluminol with IgG.
    • Testing of immunobeads, polystyrene balls, and tubes as solid-phase supports.
    • Direct and sandwich LIA formats were employed to assess conjugate properties and assay sensitivity.

    Main Results:

    Related Experiment Videos

    • Polystyrene tubes demonstrated superior linearity and reproducibility compared to other supports.
    • The sandwich LIA system achieved a sensitivity of 1 ng.
    • Conjugates exhibited stability, ease of use, and safe handling.

    Conclusions:

    • Solid-phase LIA using aminobutyl-ethylisoluminol-IgG conjugates is a viable alternative to radioimmunoassay (RIA).
    • The developed LIA system offers advantages in terms of sensitivity, cost-effectiveness, and operational safety.