Related Experiment Video
Updated: Oct 24, 2026

Dissection and Culture of Chick Statoacoustic Ganglion and Spinal Cord Explants in Collagen Gels for Neurite Outgrowth Assays
Published on: December 20, 2011
Neuritic growth and neuroma formation by an isolated molluscan ganglion
Abstract:
The isolated, paired buccal ganglia of the snail Helisoma trivolvis were cultured in vivo (i.e., in host snails) for periods up to 377 days. Such ganglia typically become enveloped in a white mass of fibrous tissue which reaches a maximum plateau of size within four weeks. The scanning electron microscope revealed the newly formed tissue to be composed of fine fibers which also pervaded the neuropil. Injection of individual neurons with Lucifer Yellow CH filled many new neurites within the tissue mass. It is concluded that these masses are largely of neuronal origin and they are therefore designated as neuromas. The maximum size of neuromas may be limited by intrinsic mechanisms which limit neuronal volume.
Related Concept Videos
Neurulation
The Neuromuscular Junction
Nervous Tissue: Myelin
Schwann cells begin to form myelin sheaths around axons during fetal development. They wrap around a small...
Neurogenesis and Regeneration of Nervous Tissue
Peripheral Nervous System: Ganglia and Nerves
Nerves
The nerve is a bundle of axons that serves as the communication highway in the PNS. Each nerve is ensheathed in a protective layer of connective tissue called the epineurium. This outermost layer safeguards the nerve and supports the...

