Related Experiment Video
Updated: Jul 19, 2026

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
Published on: July 11, 2014
High-performance liquid chromatographic determination of metoprolol in plasma
Abstract:
A high-performance liquid chromatographic method is presented for the determination of metoprolol in human plasma. Metoprolol was extracted from plasma by a single extraction procedure with 4-methylpropranolol as the internal standard. Chromatography was done on a reversed-phase column with fluorescence detection. The minimum detectable concentration was 5.0 ng/ml of plasma. The standard curve was linear in the range evaluated, 10-300 ng/ml. The within-run coefficient of variation was 2.3-6.0%, and the day-to-day variation was 6.8%. The method is free from interference by major metoprolol metabolites.
More Related Videos
13:35A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
Published on: March 1, 2018
14:39Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults
Published on: April 22, 2022
Related Concept Videos
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Elution Process
High-Performance Liquid Chromatography: Instrumentation
High-Performance Liquid Chromatography: Types of Detectors