Effect of phospholipase C on lipid peroxidation of rat liver mitochondria

Effect of phospholipase C on lipid peroxidation in rat liver mitochondria was studied. The lipid peroxidation of mitochondria in the presence of ascorbic acid and Fe2+ (FeSO4) was inhibited by approximately 45% after pretreatment of phospholipase C for 30 min. However, when phosphatidylcholine instead of mitochondria was used for the peroxidation, phospholipase C showed no inhibition on lipid peroxidation. Therefore, it may be considered that phospholipids in mitochondria are modified in diglyceride structure after digestion with phospholipase C, and the diglyceride is poor substrate for lipid peroxidation in mitochondria.