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Related Experiment Videos

Bioavailability of oestriol

D E Englund, K B Elamsson, E D Johansson

    Acta Endocrinologica
    |January 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    A new radioimmunoassay accurately measures unconjugated estriol (Oe3) in post-menopausal women. Oral estriol is rapidly absorbed but quickly metabolized, explaining its weak estrogenic effect.

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    Area of Science:

    • Endocrinology
    • Pharmacokinetics

    Background:

    • Estriol (Oe3) is a key estrogen with therapeutic applications.
    • Understanding estriol's absorption and metabolism is crucial for optimizing its use.

    Purpose of the Study:

    • To develop a rapid radioimmunoassay for unconjugated estriol analysis.
    • To investigate the pharmacokinetics of orally administered estriol in post-menopausal women.

    Main Methods:

    • Development of a specific radioimmunoassay for estriol using antiserum against Oe3.
    • Plasma samples from post-menopausal women were analyzed before and after oral estriol administration.
    • Plasma extraction with diethyl ether and subsequent radioimmunoassay without further purification.

    Main Results:

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  • Baseline plasma estriol levels in post-menopausal women were found to be very low (<5 pg/ml).
  • Oral administration of 6 mg or 12 mg estriol led to a rapid increase in plasma estriol levels within 15 minutes.
  • Plasma estriol peaked around 45 minutes and returned to low levels within 3-4 hours, indicating rapid metabolism.

    • Conclusions:

    • A sensitive radioimmunoassay for estriol analysis in plasma was successfully developed.
    • Orally administered estriol is rapidly absorbed but also rapidly metabolized.
    • The short duration of elevated plasma estriol levels likely contributes to its weak estrogenic effect when administered once daily due to low receptor affinity.