Further studies on adriamycin induced in vitro lipid peroxidation

Adriamycin (AM)-induced lipid peroxidation was studied in rat liver microsomal system, measuring colour produced by thiobarbituric acid (TBA) reactive material and the development of volatile hydrocarbons. The onset of this degradative process is closely related to the AM content. This phenomenon appears to be highly influenced by the buffer used for the microsomal preparation. The effect of AM on FE--ADP-induced lipid peroxidation in liver microsomes has also been studied.